1. Introducción
The AmScope D120A-MS is a dual-view compound monocular microscope designed for high magnification observation in biological and educational applications. It features a versatile viewing head, multiple objective lenses, Brightfield illumination, and a mechanical stage for precise specimen manipulation. This manual provides essential information for the proper setup, operation, and maintenance of your microscope.
2. Normas de seguridad
- Manipule siempre el microscopio con cuidado. Evite movimientos bruscos o impactos.
- Asegúrese de que el microscopio esté colocado sobre una superficie estable y nivelada para evitar que se vuelque.
- Connect the power cord to a grounded electrical outlet of the correct voltage (110 V).
- Do not touch optical surfaces with bare hands. Use lens paper or a soft, lint-free cloth for cleaning.
- Desconecte el microscopio de la fuente de alimentación antes de limpiarlo o realizar cualquier mantenimiento.
- Mantenga el microscopio alejado de la luz solar directa, altas temperaturas, polvo y productos químicos corrosivos.
- Supervise children or inexperienced users to ensure safe operation.
3. Contenido del paquete
Desembale con cuidado todos los componentes y verifique que se incluyan los siguientes elementos:
- AmScope D120A-MS microscope with mechanical stage
- WF10x eyepiece
- WF16x eyepiece
- 4x DIN achromatic objective
- 10x DIN achromatic objective
- 40xS DIN achromatic objective (spring-loaded)
- 100xS (oil) DIN achromatic objective (spring-loaded)
- (2) Color filters
- bombilla de repuesto
- Guardapolvo
- Manual de instrucciones (este documento)
4. Identificación de componentes
Familiarize yourself with the main parts of your AmScope D120A-MS microscope:
Figura 4.1: Encimaview of the AmScope D120A-MS microscope. This image displays the complete microscope assembly, including the base, stage, objective lenses, and dual-view monocular head.
- Eyepiece (Ocular): Hacia donde miras view the specimen. The D120A-MS includes WF10x and WF16x eyepieces.
- Monóculo Viewcabeza de ing: The upper part of the microscope containing the eyepiece tube. This model features a dual-view head with a fixed 45-degree inclined tube and a vertical port.
- Revólver portaobjetivos giratorio: Sostiene las lentes del objetivo y permite cambiar fácilmente entre aumentos.
- Lentes objetivos: The primary magnification components (4x, 10x, 40xS, 100xS oil).
- Mecánico Stage: A platform where the specimen slide is placed, with controls for precise X-Y movement.
- Stagclips electrónicos: Fije el portaobjetos de muestra en el mecanismo.tage.
- Perilla de enfoque grueso: Large knob for rapid focusing adjustments.
- Perilla de enfoque fino: Small knob for precise focusing adjustments.
- Iluminador (Fuente de luz): Ubicado debajo del stage, provides light for viewing. This model uses variable-intensity tungsten illumination.
- Condensador Abbe: Situado debajo del stage, focuses light onto the specimen. Features a 1.25 NA and an iris diaphragm.
- Diafragma del iris: Controls the amount of light passing through the specimen.
- Base: The stable bottom support of the microscope.
5. Montaje y configuración
Follow these steps to set up your microscope for initial use:
- Desembalaje: Retire con cuidado el microscopio y todos los accesorios del embalaje. Conserve el embalaje original para su posterior transporte o almacenamiento.
- Colocación: Place the microscope on a sturdy, level surface, away from vibrations and direct sunlight.
- Instalación del ocular: Insert the desired eyepiece (WF10x or WF16x) into the inclined eyepiece tube of the monocular head. Gently twist to secure it.
- Instalación objetiva: The objective lenses are typically pre-installed. If not, carefully screw each objective into the revolving nosepiece in increasing order of magnification (4x, 10x, 40xS, 100xS). Ensure they are finger-tight.
- Conexión de energía: Connect the power cord to the microscope's power input and then to a standard 110V electrical outlet.
- Guardapolvo: When not in use, cover the microscope with the provided dust cover to protect it from dust and debris.
Figura 5.1: De cerca view of the objective lenses mounted on the revolving nosepiece. This image shows the 4x, 10x, 40xS, and 100xS (oil) objectives in position.
6. Funcionamiento básico
This section outlines the fundamental steps for observing specimens with your microscope.
- Encendido: Flip the power switch located on the base of the microscope. The illuminator should light up.
- Ajustar la iluminación: Use the variable intensity control knob to set the desired brightness. Adjust the iris diaphragm on the Abbe condenser to optimize contrast and resolution for your specimen.
- Lugar de la muestra: Rotate the revolving nosepiece to select the lowest power objective (4x). Lower the mechanical stage using the coarse focus knob. Place a prepared specimen slide onto the mechanical stagy asegúrelo con la stagy clips.
- Position Specimen: Utilice los XYtage control knobs to move the slide until the area of interest is centered under the objective lens.
- Enfoque:
- While looking through the eyepiece, slowly raise the stage usando el perilla de enfoque grueso until the specimen comes into rough focus.
- Utilice el perilla de enfoque fino para lograr una imagen nítida y clara.
- Cambiar ampliación: Once focused at low power, rotate the revolving nosepiece to switch to a higher power objective (e.g., 10x, 40xS). Due to the parfocal design, only minor adjustments with the fine focus knob should be needed to bring the image back into sharp focus.
- Using the 100xS Oil Immersion Objective:
- After focusing with the 40xS objective, rotate the nosepiece halfway between the 40xS and 100xS objectives.
- Place a small drop of immersion oil directly onto the center of the specimen slide where the light passes through.
- Carefully rotate the 100xS objective into the oil drop until it clicks into place. The objective should be immersed in the oil.
- Utilice solo el perilla de enfoque fino to bring the image into sharp focus. Do not use the coarse focus knob with the 100xS objective.
- After use, clean the 100xS objective and the slide immediately with lens paper moistened with lens cleaning solution to remove all immersion oil.
- Doble-View Funcionalidad: The vertical port on the monocular head can accept a second eyepiece for simultaneous viewing by another person or a camera mount for digital image capture (eyepiece and camera sold separately). Dioptric adjustment on the upright tube accommodates individual eye-strength differences.
Figura 6.1: The AmScope D120A-MS microscope demonstrating its digital image capture capability by connecting to a laptop. This setup utilizes the vertical port for a camera attachment.
7. Mantenimiento y cuidado
Un mantenimiento adecuado garantiza la longevidad y el rendimiento óptimo de su microscopio.
- Limpieza de lentes: Use only specialized lens paper and lens cleaning solution for optical surfaces. Gently wipe in a circular motion. Never use abrasive cloths or harsh chemicals.
- Limpieza general: Limpie el cuerpo del microscopio con un paño suave y húmedo.amp Paño. Evite que la humedad entre en los componentes eléctricos.
- Almacenamiento: Always cover the microscope with the dust cover when not in use. Store it in a dry, dust-free environment.
- Reemplazo de bombilla: If the illuminator bulb burns out, unplug the microscope. Refer to the base of the microscope for access to the bulb compartment. Carefully replace the old bulb with the provided spare bulb, ensuring not to touch the glass part of the new bulb with bare hands.
- Partes mecánicas: Do not attempt to lubricate or disassemble any mechanical parts. If issues arise, contact customer support.
8. Solución de problemas
Esta sección aborda problemas comunes que podrías encontrar.
| Problema | Posible causa | Solución |
|---|---|---|
| Sin iluminación | Power cord disconnected, power switch off, bulb burnt out | Check power connection, turn on switch, replace bulb |
| La imagen está borrosa o desenfocada | Incorrect focus adjustment, objective not fully engaged, dirty lens | Adjust coarse/fine focus, rotate nosepiece until objective clicks, clean lenses |
| Campo oscuro de view | Iris diaphragm closed, illuminator too dim, condenser too low | Open iris diaphragm, increase illuminator brightness, adjust condenser height |
| Polvo o manchas en view | Dust on eyepiece, objective, or slide | Clean eyepiece, objective, or slide with lens paper |
| Cannot focus at 100x | No immersion oil, air bubbles in oil, coarse focus used | Apply immersion oil, remove air bubbles, use only fine focus |
9. Especificaciones técnicas
Detailed specifications for the AmScope D120A-MS microscope:
- Cabeza: Compound dual-view monocular, 45-degree inclined, 360-degree rotatable
- Oculares: WF10x, WF16x (with pointer)
- Objetivos: 4X, 10x, 40xS (spring), 100xS (oil, spring) DIN achromatic
- Rango de aumento: 40X - 1600X
- Stage: Mecánica stage with X-Y axis movement, stagy pare
- Configuración de iluminación: Transmitted (lower)
- Condensador: Abbe, 1.25 NA
- Diafragma: Diafragma de iris
- Fuente de luz: Tungsten (incandescent), variable intensity
- Tipo de iluminación: Campo Claro
- Fuerza: 110 V
- Material: Metal, tungsten
- Dimensiones (alto x ancho x profundidad): 15 x 7.5 x 5.125 pulgadas (aproximadamente)
- Peso: 10 libras
10. Garantía y atención al cliente
For warranty information, technical assistance, or to purchase replacement parts, please contact AmScope customer support. Refer to the AmScope website or your purchase documentation for current contact details.
